5X Phosphate Buffer, pH 8

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl) .Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4) . This buffer is the basis for wash and elution buffers for protein purification through His-tag.