Nickel NTA Magnetic Agarose Beads

Nickel NTA Magnetic Agarose Beads are a rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.NTA cross-linked resins consist of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent which provides a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.Nickel NTA Magnetic Beads are supplied as a 5% suspension of magnetic beadsin 20% ethanol (each 1 ml of suspension contains 50 μl of magnetic beads) .Loading Capacity (μmol me2+/ml gel) : >20 µmol/ ml gelBinding Capacity: >75 mg His-tagged protein/ ml gel