PKCth (Phospho-Ser676) Antibody
This is a PKCth (Phospho-Ser676) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR) -mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
Synonyms
KPCT; PKC-theta; PKCQ; PRKCQ; PRKCT
Gene ID
5588
Swiss Prot
Q04759
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 676 (R-L-S (p) -F-A) derived from Human PKCth.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
WB, IHC, IF
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
WB 1:500-1:1000, IHC 1:50-1:100, IF 1:100-1:200
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Host or Source
Rabbit
Isotype
IgG
Curated Selection
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