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PGK1 (Acetyl-Lys388) Antibody

This is a PGK1 (Acetyl-Lys388) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) .

Synonyms

MGC117307; MGC142128; MGC8947; MIG10; PGKA

Gene ID

5230

Swiss Prot

P00558

Reactivity

Human, Mouse

Immunogen

Peptide sequence around acetylation site of lysine 388 (E-D-K (Acetyl) -V-S) derived from Human PGK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB, IHC, IP

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000, IHC 1:50-1:100, IP 1:20-1:50

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Calculated Molecular Weight

45kDa

Host or Source

Rabbit

Isotype

IgG

Curated Selection

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