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P47 phox (Phospho-Ser345) Antibody

This is a p47 phox (Phospho-Ser345) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production) .

Synonyms

NCF1; P47 phox; NCF-47K

Gene ID

653361

Swiss Prot

P14598

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of Serine 345 (P-Q-S (p) -P-G) derived from Human Nuclear p47 phox.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB, IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000, IHC 1:50-1:100

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Observed Molecular Weight

45kDa

Host or Source

Rabbit

Isotype

IgG

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