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B-Raf (Phospho-Thr753) Antibody

This is a B-Raf (Phospho-Thr753) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Synonyms

B-RAF; RAFB1; RMIL; p94

Gene ID

673

Swiss Prot

P15056

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of threonine 753 (P-K-T (p) -P-L) derived from Human B-Raf.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Observed Molecular Weight

84kDa

Host or Source

Rabbit

Isotype

IgG

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