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INOS (Phospho-Tyr151) Antibody

This is a iNOS (Phospho-Tyr151) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Synonyms

HEP-NOS; NOS2; inducible

Gene ID

4843

Swiss Prot

P35228

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 151 (Q-Y-Y (p) -G-S) derived from Human iNOS.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

IHC 1:50-1:100

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Observed Molecular Weight

131kDa

Host or Source

Rabbit

Isotype

IgG

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