PEK/PERK (Phospho-Thr982) Antibody
This is a PEK/PERK (Phospho-Thr982) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR) -induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) .
Synonyms
E2AK3; PERK; PEK; EIF2AK3
Gene ID
9451
Swiss Prot
Q9NZJ5
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 982 (R-H-T (p) -G-Q) derived from Human PEK/PERK.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
IHC
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
IHC 1:50-1:100
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Observed Molecular Weight
125kDa
Host or Source
Rabbit
Isotype
IgG