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CK-1ɑ (Phospho-Tyr294) Antibody

This is a CK-1ɑ (Phospho-Tyr294) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Synonyms

KC1A; CKI-alpha; CSNK1A1; alpha isoform; Casein kinase I

Gene ID

1452

Swiss Prot

P48729/Q8N752

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y (p) -T-F) derived from Human CK-1ɑ.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB, IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000, IHC 1:50-1:100

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Observed Molecular Weight

37kDa

Host or Source

Rabbit

Isotype

IgG

Curated Selection

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