CK-1ɑ (Phospho-Tyr294) Antibody
This is a CK-1ɑ (Phospho-Tyr294) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.
Synonyms
KC1A; CKI-alpha; CSNK1A1; alpha isoform; Casein kinase I
Gene ID
1452
Swiss Prot
P48729/Q8N752
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y (p) -T-F) derived from Human CK-1ɑ.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
WB, IHC
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Observed Molecular Weight
37kDa
Host or Source
Rabbit
Isotype
IgG
Curated Selection
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