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PDK1 (Phospho-Tyr9) Antibody

This is a PDK1 (Phospho-Tyr9) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.

Synonyms

HPDK1; PDPK1; mPDK1; PkB kinase

Gene ID

5170

Swiss Prot

O15530

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 9 (A-L-Y (p) -D-A) derived from Human PDK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

IHC 1:50-1:100

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Observed Molecular Weight

63kDa

Host or Source

Rabbit

Isotype

IgG

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