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HSF1 (Phospho-Thr142) Antibody

This is a HSF1 (Phospho-Thr142) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Synonyms

HSF 1; HSTF1; Heat shock factor protein 1

Gene ID

3297

Swiss Prot

Q00613

Reactivity

Human, Mouse

Immunogen

Peptide sequence around phosphorylation site of threonine 142 (L-L-T (p) -D-V) derived from Human HSF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

IHC 1:50-1:100

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Observed Molecular Weight

57kDa

Host or Source

Rabbit

Isotype

IgG

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