ENOS (Phospho-Thr495) Antibody
This is a eNOS (Phospho-Thr495) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Synonyms
CNOS; EC-NOS; ECNOS; NOS; NOS3
Gene ID
4846
Swiss Prot
P29474
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 495 (K-K-T (p) -F-K derived from Human eNOS.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
WB
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
WB 1:500-1:1000
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Observed Molecular Weight
140kDa
Host or Source
Rabbit
Isotype
IgG
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