WEE1 (Phospho-Ser642) Antibody

This is a WEE1 (Phospho-Ser642) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Synonyms

WEE1A; WEE1hu; kinase WEE1

Gene ID

7465

Swiss Prot

P30291

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of Serine 642 (S-V-S (p) -L-T) derived from Human WEE1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.