Bloom Syndrome (Phospho-Thr99) Antibody
This is a Bloom Syndrome (Phospho-Thr99) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE) .
Synonyms
RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3
Gene ID
641
Swiss Prot
P54132
Reactivity
Human
Immunogen
Peptide sequence around phosphorylation site of threonine 99 (Q-E-T (p) -Q-R) derived from Human Bloom Syndrome.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
WB, IHC
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Observed Molecular Weight
159kDa
Host or Source
Rabbit
Isotype
IgG
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