Ras-GRF1 (Phospho-Ser916) Antibody
This is a Ras-GRF1 (Phospho-Ser916) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors) . Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist) -induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.
Synonyms
CDC25; GNRP; GRF1; RASGRF1
Gene ID
5923
Swiss Prot
Q13972
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Serine 916 (R-M-S (p) -L-A) derived from Mouse Ras-GRF1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
IHC
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
IHC 1:50-1:100
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Observed Molecular Weight
145kDa
Host or Source
Rabbit
Isotype
IgG
Curated Selection
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