Ras-GRF1 (Phospho-Ser916) Antibody

This is a Ras-GRF1 (Phospho-Ser916) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors) . Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist) -induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Synonyms

CDC25; GNRP; GRF1; RASGRF1

Gene ID

5923

Swiss Prot

Q13972

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of Serine 916 (R-M-S (p) -L-A) derived from Mouse Ras-GRF1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.