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MLC2 (Phospho-Tyr118) Antibody

This is a MLC2 (Phospho-Tyr118) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

Synonyms

MLC2; MRLC1; MYRL2

Gene ID

4633

Swiss Prot

P10916

Reactivity

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 118 (A-D-Y (p) -V-R) derived from Human MLC2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB, IF

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000, IF 1:100-1:200

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Observed Molecular Weight

20kDa

Host or Source

Rabbit

Isotype

IgG

Frequently Asked Questions

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