EIF2a (Phospho-Ser51) Antibody
This is a eIF2a (Phospho-Ser51) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Synonyms
Eukaryotic translation initiation factor 2 subunit alpha; EIF-2A
Gene ID
1965
Swiss Prot
P05198
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 51 (E-L-S (p) -R-R) derived from Human eIF2a.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
WB, IHC, IF
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
IF 1:50-1:200, WB 1:500-1:2000, IHC 1:100-1:300
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Host or Source
Rabbit
Isotype
IgG
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