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IkB-e (Phospho-Ser22) Antibody

This is a IkB-e (Phospho-Ser22) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Synonyms

I-kappa-B-epsilon; IKBE; IkappaBepsilon; NF-kappa-BIE; NF-kappaB inhibitor epsilon

Gene ID

18037

Swiss Prot

O54910

Reactivity

Human, Mouse

Immunogen

Peptide sequence around phosphorylation site of serine 22 (I-E-S (p) -L-R) derived from Mouse IkB-ε.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB, IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000, IHC 1:50-1:100

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Host or Source

Rabbit

Isotype

IgG

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