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Histone H3.1 (Phospho-Ser10) Antibody

This is a Histone H3.1 (Phospho-Ser10) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Synonyms

H3/b; H3FB

Gene ID

8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968

Swiss Prot

P68431

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of serine 10 (R-K-S (p) -T-G) derived from Human Histone H3.1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB, IHC, IF

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000, IHC 1:50-1:100, IF 1:100-1:200

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Observed Molecular Weight

17kDa

Host or Source

Rabbit

Isotype

IgG

Curated Selection

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