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ENOS (Phospho-Ser1177) Antibody

This is a eNOS (Phospho-Ser1177) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Constitutive NOS; EC-NOS; ECNOS; NOS3; NOSIII

Gene ID

4846

Swiss Prot

P29474

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of serine 1177 (T-Q-S (p) -F-S) derived from Human eNOS.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Host or Source

Rabbit

Isotype

IgG

Curated Selection

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