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Cdc25A (Phospho-Ser76) Antibody

This is a cdc25A (Phospho-Ser76) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Synonyms

CDC25M3; MPIP1

Gene ID

993

Swiss Prot

P30304

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of serine 76 (M-G-S (p) -S-E) derived from Human cdc25A.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB, IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000, IHC 1:50-1:100

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Host or Source

Rabbit

Isotype

IgG

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