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Akt2 (Phospho-Ser474) Antibody

This is a Akt2 (Phospho-Ser474) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

Product Specifications

Background

General protein kinase capable of phosphorylating several known proteins.

Synonyms

PKB beta; Protein kinase B; RAC-PK-beta

Gene ID

208

Swiss Prot

P31751

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence around phosphorylation site of serine 474 (Q-F-S (p) -Y-S) derived from Human Akt2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Phospho-Specific Antibody

Applications

WB, IHC

Purification Method

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Assay Type

Antibody

Concentration

1.0 mg/mL

Dilution

WB 1:500-1:1000, IHC 1:50-1:100

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Conditions

Store at -20°C. Avoid freeze / thaw cycles.

Host or Source

Rabbit

Isotype

IgG

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