Raf1 (Phospho-Ser259) Antibody
This is a Raf1 (Phospho-Ser259) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
Synonyms
C-RAF; C-Raf; CRAF
Gene ID
5894
Swiss Prot
P04049
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 259 (S-T-S (p) -T-P) derived from Human Raf1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
WB, IHC
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
WB 1:500-1:1000, IHC 1:50-1:100
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Host or Source
Rabbit
Isotype
IgG
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