Histone H3 (Di-Methyl-Lys27) Antibody
This is a Histone H3 (Di-Methyl-Lys27) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.
Product Specifications
Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms
H3/a; H3/c; H3/d; H3/f; H3/h
Gene ID
8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
Swiss Prot
P68431
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around Di-Methylation site of lysine 27 (A-R-K (di-methyl) -S-A) derived from Human Histone H3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Phospho-Specific Antibody
Applications
WB, IHC
Purification Method
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Assay Type
Antibody
Concentration
1.0 mg/mL
Dilution
IHC 1:50-1:100
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Conditions
Store at -20°C. Avoid freeze / thaw cycles.
Observed Molecular Weight
17kDa
Host or Source
Rabbit
Isotype
IgG
Curated Selection
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