Eosin Y
Hematoxylin and Eosin (H&E) staining is used most commonly in histology.
Product Specifications
Applications
IHC
Assay Principle
H and E staining: Follow the procedure as outlined for H and E staining. We use the following protocol:
1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections.
2. Stain with hematoxylin for 4 minutes (2X), wash 4-5X with tap water, and rinse 2 X with de/di water.
3. Dip in 10 % acetic acid 2X, wash with de/di water, wash with Immuno Automation buffer (ImmunoBioScience AR-6561), PBS or any buffer with pH 7.4-8.
4. Rinse 2X with 70% ethanol, apply Eosin stain 1-5 minutes, and wash with 70% ethanol to remove excess Eosin..
5. Rinse 2X with 95% ethanol, 2X with 100% ethanol, xylene or any dehydrating agent.
6. The slide can be mounted with organic mounting medium or our Organo /limonene (AR-6504) mounting medium.
Shipping Conditions
Ambient Temperature
Storage Conditions
Room Temperature
Curated Selection
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