Biotinylated SARS-CoV-2 Spike S2 protein, His, Avitag™ (BA.2/Omicron)

Biotinylated SARS-CoV-2 Spike S2 protein, His, Avitag™ (BA.2/Omicron) (S2N-C82E4) is expressed from human 293 cells (HEK293) . It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1 (N764K, D796Y, Q954H, N969K, F817P, A892P, A899P, A942P, K986P, V987P) ) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2/3/4/5) . Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production.

Product Specifications

Background

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 61.7 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

QHD43416.1 (N764K, D796Y, Q954H, N969K, F817P, A892P, A899P, A942P, K986P, V987P)

Expression Region

Ser 686 - Pro 1213

Host

HEK293

Target

Spike S2

Conjugation

Biotin-labeled

Tag

C-10xHis & C-Avi

Source

SARS-CoV-2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag (BA.2/Omicron) (Cat. No. S2N-C82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike S2 protein Antibody, Human IgG4 (Cat. No. S2N-S86) with a linear range of 0.1-1 ng/mL (QC tested).