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SARS-CoV-2 Spike Trimer Protein, His Tag (NB.1.8.1) (MALS verified)

SARS-CoV-2 Spike Trimer Protein, His Tag (NB.1.8.1) (SPN-S522b) is expressed from human 293 cells (HEK293) . It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, R21T, T22N, A27S, S50L, F59S, V127F, G142D, F157S, R158G, G184S, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, A435S, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478I, N481K, E484K, F486P, Q493E, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, F817P, A892P, A899P, A942P, K986P, V987P, D796Y, S939F, Q954F, N969K, P1143L) ) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein.

Product Specifications

Background

SARS‑CoV‑2 NB.1.8.1 is an Omicron‑descendant variant under monitoring, first detected in early 2025. It carries multiple spike mutations linked to immune escape and shows a growth advantage over co‑circulating BA.2.86/JN.1 lineages2. While currently at low prevalence in many regions, it is spreading in parts of Asia and is expected to rise, though no major increase in disease severity is anticipated.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 138.6 kDa. The protein migrates as 80-90 kDa and 100-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Expression Region

Val 16 - Pro 1213

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Format

Powder

Buffer

0.1 M Sodium citrate

Molecular Weight

138.6 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

100ug*1

Product Datasheet

https://console.acrobiosystems.com/public/product/pdf/ds/SPN-S522b.pdf

Host or Source

HEK293

Species

SARS-CoV-2

Protein ID

QHD43416.1 (T19I, R21T, T22N, A27S, S50L, F59S, V127F, G142D, F157S, R158G, G184S, L212I, V213G, L216F, H245N, A264D, I332V, G339H, K356T, S371F, S373P, S375F, T376A, R403K, D405N, R408S, K417N, A435S, N440K, V445H, G446S, N450D, L452W, L455S, F456L, N460K, S477N, T478I, N481K, E484K, F486P, Q493E, Q498R, N501Y, Y505H, E554K, A570V, D614G, P621S, H655Y, N679K, P681R, N764K, F817P, A892P, A899P, A942P, K986P, V987P, D796Y, S939F, Q954F, N969K, P1143L)

Preservative

Trehalose

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