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Bat coronavirus RS4081 Spike Trimer Protein (R653A, K954P, V955P), His Tag (MALS verified)

The spike protein of bat coronavirus RS4081, a sarbecovirus related to SARS-CoV, mediates viral entry by facilitating attachment and membrane fusion. RS4081’s spike can support ACE2-independent, trypsin-dependent cell entry, highlighting alternative pathways for host infection and its potential relevance to zoonotic spillover and cross-species transmission studies.

Product Specifications

Background

The spike protein of bat coronavirus RS4081, a sarbecovirus related to SARS-CoV, mediates viral entry by facilitating attachment and membrane fusion. RS4081’s spike can support ACE2-independent, trypsin-dependent cell entry, highlighting alternative pathways for host infection and its potential relevance to zoonotic spillover and cross-species transmission studies.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 134.9 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Expression Region

Gln 16 - Pro 1181

Target

Spike protein

Conjugation

Unconjugated

Tag

C-10xHis

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Format

Powder

Buffer

0.1 M Sodium citrate, pH5.5

Molecular Weight

134.9 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

500ug*2

Host or Source

HEK293

Species

Bat coronavirus RS4081

Protein ID

ATO98120.1 (R653A, K954P, V955P)

Preservative

Trehalose

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