TRAIL R2 / CD262 / TNFRSF10B Protein (His Tag)

Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.

Product Specifications

Synonyms

CD262 Protein; DR5 Protein; KILLER Protein; KILLER/DR5 Protein; TRAIL-R2 Protein; TRAILR2 Protein; TRICK2 Protein; TRICK2A Protein; TRICK2B Protein; TRICKB Protein; UNQ160/PRO186 Protein; ZTNFR9 Protein

Accession Number

NP_003833.4

Reactivity

Human

Cross Reactivity

Human

Label

His-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1. Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10B at 10 μg/mL (100 μL/well) can bind biotinylated TNFSF10 with a linear range of 0.625-20 ng/mL. 2. Loaded Recombinant Human DR5/TRAIL R2 Protein, His Tag on HIS1K Biosensor, can bind Recombinant Human TRAIL/TNFSF10 Protein with an affinity constant of 0.122 nM as determined in BLI assay (Sartorius Octet RED384) .

Length

138

Form

Lyophilized or frozen liquid

Buffer

PBS, pH 7.4

Reconstitution

See Datasheet

Molecular Weight

22 kDa (glycosylated)

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.