TIMP-3

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Product Specifications

Synonyms

TIMP3; SFD; K222; K222TA2; HSMRK222

NCBI Gene ID

7078

UniProt

P35625

Accession Number

NP_000353

Accession Number mRNA

NM_000362

Chromosomal Location

22q12.1-q13.2;22q12.3

Reactivity

Anti-Human

Cross Reactivity

Human

Target Antigen

Human recombinant TIMP-3 protein

Clone

(#7D52)

Applications

WB, IHC (P)

Purification Method

Protein G chromatography

Assay Protocol

Centrifuge vial prior to opening. Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Form

Lyophilized

Buffer

PBS

Reconstitution

PBS

Storage Conditions

Lyophilized samples are stable for 2 years from date of receipt when stored at -20°C. Reconstituted antibody can be aliquoted and stored frozen at < -20°C for at least six months without detectable loss of activity.