Thrombopoietin / THPO Protein (hFc Tag)

Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.

Product Specifications

Synonyms

CAMT2 Protein; MGDF Protein; MKCSF Protein; ML Protein; MPLLG Protein; THC9 Protein; THCYT1 Protein; TPO Protein

Accession Number

NP_000451.1

Reactivity

Human

Cross Reactivity

Human

Label

HFc-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Recombinant Human TPO/Thrombopoietin Protein, hFc Tag captured on Protein A chip can bind Recombinant Human TPO-R Protein, His Tag with an affinity constant of 12.3 nM as determined in an SPR assay (Biacore 8K) .

Length

409

Form

Lyophilized or frozen liquid

Buffer

PBS, pH 7.4

Reconstitution

See Datasheet

Molecular Weight

45 kDa

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.