TGF-alpha

TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-α induces transformation anchorage independence in cultured cells. Human, murine and rat TGF-α are cross-species reactive. Recombinant human TGF-α is a 50 amino acid polypeptide (5.5 kDa) which shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

Product Specifications

Synonyms

TGFA; TFGA

NCBI Gene ID

7039

UniProt

P01135

Accession Number

NP_003227.1

Accession Number mRNA

NM_003236

Chromosomal Location

2p13

Reactivity

Human

Cross Reactivity

Chicken, Mouse, Rat, Human, Horse

Sequence

VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 106 units/mg.