SCGF-beta

SCGF-α and -β are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Recombinant human SCGF-β is a 25.0 kDa polypeptide containing 227 amino acid residues.

Product Specifications

Synonyms

CLEC11A; P47; SCGF; LSLCL; CLECSF3

NCBI Gene ID

6320

UniProt

Q9Y240

Accession Number

NP_002966.1

Accession Number mRNA

NM_002975.2

Chromosomal Location

19q13.3

Reactivity

Human

Cross Reactivity

Human

Sequence

MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells, previously grown in media containing GM-CSF. The expected ED50 for this effect is ≤ 7.0 ng/ml.