Prolactin
Recombinant ovine prolactin, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (see Leibovich et al., Protein Expr Purif. 2001 22:489-96) .
Product Specifications
Synonyms
Mammotropin, Luterotropic hormone, Lutetropin
NCBI Gene ID
443317
UniProt
P01240
Accession Number
NP_001009306.1
Accession Number mRNA
NM_001009306.1
Reactivity
Ovine
Cross Reactivity
Ovine
Sequence
ATPVCPNGPGNCQVSLRDLFDRAVMVSHYIHNLSSEMFNEFDKRYAQGKGFITMALNSCHTSSLPTPEDKEQAQQTHHEVLMSLILGLLRSWNDPLYHLVTEVRGMKGVPDAILSRAIEIEEENKRLLEGMEMIFGQVIPGAKETEPYPVWSGLPSLQTKDEDARHSAFYNLLHCLRRDSSKIDTYLKLLNCRIIYNNNC
Assay Protocol
It is recommended to reconstitute the lyophilized oPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 99% by SDS-PAGE & HPLC analyses
Bioactivity
Recombinant oPRL is fully biologically active as evidenced by inducing proliferation of Nb2 cells.
Length
199
Form
Lyophilized
Reconstitution
Water
Molecular Weight
23.0 kDa
Host or Source
E. coli
N Terminal Sequence
ATPVCP
More Discoveries
Explore Other Products
Browse additional items from our catalog