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Prolactin

Recombinant ovine prolactin, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (see Leibovich et al., Protein Expr Purif. 2001 22:489-96) .

Product Specifications

Synonyms

Mammotropin, Luterotropic hormone, Lutetropin

NCBI Gene ID

443317

UniProt

P01240

Accession Number

NP_001009306.1

Accession Number mRNA

NM_001009306.1

Reactivity

Ovine

Cross Reactivity

Ovine

Sequence

ATPVCPNGPGNCQVSLRDLFDRAVMVSHYIHNLSSEMFNEFDKRYAQGKGFITMALNSCHTSSLPTPEDKEQAQQTHHEVLMSLILGLLRSWNDPLYHLVTEVRGMKGVPDAILSRAIEIEEENKRLLEGMEMIFGQVIPGAKETEPYPVWSGLPSLQTKDEDARHSAFYNLLHCLRRDSSKIDTYLKLLNCRIIYNNNC

Assay Protocol

It is recommended to reconstitute the lyophilized oPRL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 99% by SDS-PAGE & HPLC analyses

Bioactivity

Recombinant oPRL is fully biologically active as evidenced by inducing proliferation of Nb2 cells.

Length

199

Form

Lyophilized

Reconstitution

Water

Molecular Weight

23.0 kDa

Host or Source

E. coli

N Terminal Sequence

ATPVCP

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