Prokineticin-2

Prokineticin-2 (PK2) is a cysteine-rich secreted protein that is expressed in the testis and in lower levels of the small intestine. PK2 regulates various biological functions including gastrointestinal motility, angiogenesis and circadiam rhythms. It is closely related to EG-VEGF (Prokineticin-1) and binds to two orphan B-protein-coupled receptors termed PK-R1 and PK-R2. Recombinant human Prokineticin-2 is an 8.8 kDa protein consisting of 81 amino acid residues and ten cysteine residues that potentially form five pairs of intra-molecular disulfide bonds.

Product Specifications

Synonyms

PROK2

NCBI Gene ID

60675

UniProt

Q9HC23

Accession Number

NP_068754.1

Accession Number mRNA

NM_021935.3

Chromosomal Location

3p13

Reactivity

Human

Cross Reactivity

Mouse, Rat, Human

Sequence

AVITGACDKD SQCGGGMCCA VSIWVKSIRI CTPMGKLGDS CHPLTRKVPF FGRRMHHTCP CLPGLACLRT SFNRFICLAQ K

Assay Protocol

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

Data not available.

Length

Form

Lyophilized

Molecular Weight

8.8 kDa

Storage Conditions

The lyophilized protein is stable at room temperature for 1 month, at 4°C for 6 months and at -20 to -80°C up to December 2019. Reconstituted working aliquots are stable for 1 week at 2°C to 8°C and for 3 months at -20°C to -80°C.