PD-L1 / B7-H1 / CD274 Protein (ECD, mFc Tag)

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant Human PD1-His at 10 μg/mL (100 μL/well) can bind human PD-L1 with a linear range of 1.28-20 μg/mL. 2. Labeled biotin to PD-L1 Protein, Human, Recombinant (ECD, Fc Tag) by a certain molar ratio; using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (ECD, Fc Tag), Biotinylated bound to Atezolizumab was 0.1 nM. 3. CHO cells were transduced with human PD1 and subjected to flow cytometric analysis using recombinant human PDL1 protein (mouse IgG2a Fc tag) and a negative control protein. The cells were then stained with an FITC-conjugated anti-mouse IgG2a Fc antibody. Non-transduced CHO cells were used as a control. 4. Immobilized Recombinant Human PD-L1/B7-H1 Protein (ECD, mFc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-1 Protein (ECD, hFc Tag) the EC50 is 67-201 ng/mL. 5. Serial dilutions of Nivolumab were added into Recombinant Human PD-L1 / B7-H1 / CD274 Protein (ECD, mFc Tag), the Nivolumab derived Anti-PD1 Antibody, Human IgG4 can block the binding of 20 μg/mL of Recombinant Human PD-1 Protein (ECD), Biotinylated to Recombinant Human PD-L1 / B7-H1 / CD274 Protein (ECD, mFc Tag) . The IC50 is 278.7 ng/mL.

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.