MSLN / Mesothelin Protein (aa 296-580, hFc Tag)

The megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised of several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level) . It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.

Product Specifications

Synonyms

CAK1 Protein; MPF Protein; SMRP Protein

Accession Number

Q13421-2

Reactivity

Human

Cross Reactivity

Human

Label

HFc-Tag

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Immobilized Recombinant Human MSLN / Mesothelin Protein (aa 296-580, Fc Tag) at 2 μg/mL (100 μL/well) can bind Anti-MSLN Antibody, human IgG1, the EC50 is 10-30 ng/mL.

Length

545

Form

Lyophilized

Buffer

PBS, pH 7.4

Reconstitution

See Datasheet

Molecular Weight

65 kDa

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.