MIF
Macrophage Migration Inhibitory Factor (MIF) is a small secreted protein that can act as a pleiotropic pro-inflammatory cytokine as well as an enzyme. MIF pro-inflammatory activity can be initiated by signaling through CD74 and CD44, resulting in the secretion of TNF-α, IL-1, IL-6, IL-8, and various MMPs. The enzymatic activity of MIF is characterized by its ability to act as a tautomerase, capable of catalyzing the keto to enol isomerization of keto-phenylpyruvate and L-dopachrome. It appears as though MIF catalytic activity is dependent upon a trimeric configuration and a free N-terminal proline residue. Insect cell derived recombinant MIF is a 15 kDa protein containing 124 amino acid residues, including an N-terminal His-tag.
Product Specifications
Synonyms
Macrophage migration Inhibitory factor, GLIF, MMIF, GIF, Glycosylation-inhibiting factor
NCBI Gene ID
4282
UniProt
P14174
Accession Number
NP_002406.1
Accession Number mRNA
NM_002415.1
Chromosomal Location
22q11.23
Reactivity
Human
Cross Reactivity
Human
Label
His-Tag
Sequence
HHHHHHHHAM PMFIVNTNVP RASVPDGFLS ELTQQLAQAT GKPPQYIAVH VVPDQLMAFG GSSEPCALCS LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA
Assay Protocol
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98% by SDS-PAGE & HPLC analyses
Bioactivity
Determined by its ability to inhibit monocyte migration.
Length
124
Form
Lyophilized
Buffer
10mM Sodium Citrate, pH 3.0
Reconstitution
Water
Molecular Weight
15 kDa
Storage Conditions
Host or Source
Insect cells
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
