Leptin antagonist (mutant L39A/D40A/F41A)
Recombinant ovine leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa, oLEP was mutated, resulting in L39A/D40A/F41A mutant was purified by proprietary chromatographic techniques. Preparation of recombinant leptin antagonists was recently published - Niv-Spector et al, Biochem. J 291;221-230 (2005) .
Product Specifications
Synonyms
Lep; ob; obese
NCBI Gene ID
443534
UniProt
G8BLB2
Accession Number
XP_027824581.2
Accession Number mRNA
XM_027968780.2
Reactivity
Ovine
Cross Reactivity
Ovine
Sequence
AVPIRKVQDDTKTLIKTIVTRINDISHTQSVSSKQRVTGAAAIPGLHPLLSLSKMDQTLAIYQQILASLPSRNVIQISNDLENLRDLLHLLAASKSCPLPQVRALESLESLGVVLEASLYSTEVVALSRLQGSLQDMLRQLDLSPG
Assay Protocol
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
Length
146
Form
Lyophilized
Reconstitution
Water
Molecular Weight
16.0 kDa
Host or Source
E. coli
N Terminal Sequence
AVPIR
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