Leptin antagonist (mutant L39A/D40A/F41A)
Recombinant rat leptin, one polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 16 kDa. Recombinant rat leptin was mutated, resulting in L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques, according to Salomon et al (2006) Protein Expression and PuriWcation 47, 128–136.
Product Specifications
Synonyms
Lep; ob; obese
NCBI Gene ID
25608
UniProt
P50596
Accession Number
NP_037208
Accession Number mRNA
NM_013076
Reactivity
Rat
Cross Reactivity
Rat
Sequence
AVPIHKVQDDTKTLIKTIVTRINDISHTQSVSARQRVTGAAAIPGLHPILSLSKMDQTLAVYQQILTSLPSQNVLQIAHDLENLRDLLHLLAFSKSCSLPQTRGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDLSPEC
Assay Protocol
It is recommended to reconstitute the lyophilized recombinant rat leptin antagonist in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
Recombinant rat leptin antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.
Length
146
Form
Lyophilized
Reconstitution
Water
Molecular Weight
16.0 kDa
Host or Source
E. coli
N Terminal Sequence
AVPIQ
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