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Lactogen, placental

Recombinant ovine placental lactogen, one polypeptide chain containing 199 amino and an additional Ala at N-terminus acids and having a molecular mass of ~ 23 kDa, was purified by proprietary chromatographic techniques (see Sakal et al. J. Endocrinology (1997), 152, 317-327. Ovine PL is also capable of activating human and other heterologous GH receptors but not ruminat GH receptors.

Product Specifications

Synonyms

Chorionic somatomammotropin hormone, Placental lactogen (PL)

NCBI Gene ID

443319

UniProt

P16038

Accession Number

NP_001009309.2

Accession Number mRNA

NM_001009309.4

Reactivity

Ovine

Cross Reactivity

Ovine

Sequence

AQHPPYCRNQPGKCQIPLQSLFDRATTVANYNSKLAGEMVNRFDEQYGQGINSESKVINCHTSSITTPNSKAEAINTEDKILFKLVISLLHSWDEPLHHAVTELANSKGTSPALLTKAQEIKEKAKVLVDGVEVIQKRIHPGEKNEPYPVWSEQSSLTSQDENVRRVAFYRLFHCLHRDSSKIYTYLRILKCRLTSCET

Assay Protocol

It is recommended to reconstitute the lyophilized oPL in sterile water or 0.4% NaHCO3 adjusted tp pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 99.0% as determined by Gel filtration and SDS-PAGE gel.

Bioactivity

Recombinant oPL is fully biologically active as evidenced by inducing proliferation of Nb2 cells.

Length

199

Form

Lyophilized

Reconstitution

Water

Molecular Weight

23.0 kDa

Host or Source

E. coli

N Terminal Sequence

AQHPP

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