IL-28B

IL-28B is a cytokine remotely related to type I interferons and the IL-10 family. Interleukin-28B, interleukin 28A (IL28A), and interleukin 29 (IL29) are 3 closely related cytokine genes which form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by these 3 genes can be induced by viral infection. All 3 cytokines interact with a heterodimeric class II cytokine receptor which consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA) .

Product Specifications

Synonyms

Interferon lambda-3, IFN-lambda-3, Interleukin-28B, IL-28B, Ifnl3, Il28, Il28b

NCBI Gene ID

338374

UniProt

Q8CGK6

Accession Number

NP_796370.1

Accession Number mRNA

NM_177396.1

Reactivity

Mouse

Cross Reactivity

Mouse

Sequence

DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKGAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENINDS ALTTILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFQLL LRDLKCVASG DQCV

Assay Protocol

It is recommended to reconstitute the lyophilized IL-28B in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 97% by SDS-PAGE & HPLC analyses

Bioactivity

The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x 104 IU/mg.