IL-22

IL-22 is a member of the IL-10 family of regulatory cytokines which includes IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26. Members of this family share partial homology in their amino acid sequences, but they are dissimilar in their biological functions. Produced by T lymphocytes, IL-22 inhibits IL-4 production by Th2 cells, and induces acute phase reactants in the liver and pancreas. IL-22 signals through a receptor system consisting of IL-10R-β/CRF2-4 and IL-22R, both of which are members of the class II cytokine-receptor family. Recombinant human IL-22 is a 33.6 kDa non-disulfide-linked homodimeric protein containing of two 147 amino acid polypeptide chains.

Product Specifications

Synonyms

Interleukin-22, IL-TIF, Cytokine ZCYTO18

NCBI Gene ID

50616

UniProt

Q9GZX6

Accession Number

NP_065386.1

Accession Number mRNA

NM_020525.4

Chromosomal Location

12q15

Reactivity

Human

Cross Reactivity

Monkey, Human

Sequence

MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

Assay #1: Determined by its ability to stimulate the proliferation of HEK-Blue™ IL-22 reporter cells. Assay #2: Measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells) . The expected ED50 for this effect is 0.7- 1.0 ng/ml.