IL-1 alpha

IL-1α is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant rat IL-1α is a 17.7 kDa protein containing 155 amino

Product Specifications

Synonyms

Il1a; IL-1 alpha

NCBI Gene ID

24493

UniProt

P16598

Accession Number

NP_058715.1

Accession Number mRNA

NM_017019

Chromosomal Location

3q36

Reactivity

Rat

Cross Reactivity

Mouse, Rat

Sequence

MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS

Assay Protocol

Reconstitute in0.5X PBS to a concentration of 0.1-1.0mg/mL.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98% by SDS-PAGE & HPLC analyses

Bioactivity

The ED50 as determined by the dose-dependent proliferation of murine D10S cells is < 0.005 ng/ml, corresponding to a specific activity of > 2 x 108 units/mg.