IGF-1
IGF-1 is a small protein secreted mainly but not solely by the liver and circulating in blood mostly as a complex with various IGF binding proteins in the blood. It has growth-regulating, insulin-like, and mitogenic activities and it is secreted in response to growth hormone stimulation. Recombinant rat IGF-I produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 71 amino acids and having a molecular mass of 7.6 kDa.
Product Specifications
Synonyms
Igf1; Igf-1; Igf-I; C730016P09Rik
NCBI Gene ID
24482
UniProt
P08025
Accession Number
NP_001075948.1
Accession Number mRNA
NM_001082479.1
Reactivity
Rat
Cross Reactivity
Rat
Sequence
MGPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKSA
Assay Protocol
It is recommended to reconstitute the lyophilized rIGF-I in sterile 0.4% NaHCO3 adjusted, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
>98.0% as determined by SDS-PAGE
Bioactivity
The ED50 as determined by the stimulation of protein synthesis in rat L6 myoblasts was found to be < 30ng/ml corresponding to a Specific Activity of 33,334IU/mg.
Length
71
Form
Lyophilized
Reconstitution
0.4% NaHCO3
Molecular Weight
7.6 kDa
Host or Source
E. coli
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
IRBAMLITPR2AAP051120UL 120 µL
Rabbit Anti ITPR2 Polyclonal Antigen affinity Purified (PBS with 0.05% NaN3 and 40% Glycerol, pH7.4) (IHC,ELISA) 120ul
Sign In for Pricing
View Details 