IFN beta (Animal Free)

Proteins of this family play an important role in inducing non-specific resistance against a broad range of viral infections. They also affect cell proliferation and modulate immune responses. Produced by peripheral blood leukocytes and lymphoblastoid cells, IFN-α is an acid-stable molecule that signals through IFN-α/βR, which is also used by IFN-β. Both IFNs have similar anti-viral activity and regulate expression of MHC class I antigens. IFN-α contains four highly conserved cysteine residues that form two disulfide bonds, one of which is necessary for biological activity. Recombinant Human IFN-β is a 20.0 kDa protein containing 166 amino acid residues.

Product Specifications

Synonyms

IFNB1; IFB; IFF; IFNB

NCBI Gene ID

3456

UniProt

P01574

Accession Number

NP_002167.1

Accession Number mRNA

NM_002176

Chromosomal Location

9p21

Reactivity

Human

Cross Reactivity

Human, Mouse

Sequence

MSYNLLGFLQ RSSNFQCQKL LWQLNGRLEY CLKDRMNFDI PEEIKQLQQF QKEDAALTIY EMLQNIFAIF RQDSSSTGWN ETIVENLLAN VYHQINHLKT VLEEKLEKED FTRGKLMSSL HLKRYYGRIL HYLKAKEYSH CAWTIVRVEI LRNFYFINRL TGYLRN

Endotoxin

< 0.01 ng/µg of protein (< 0.1 EU/µg)

Purity

≥ 95% by SDS-PAGE gel and HPLC analyses.

Bioactivity

Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.25 ng/ml, corresponding to a specific activity of ≥ 4 x 106 units/mg.