HGF Protein

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain, and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. The hepatocyte growth factor is secreted by mesenchymal cells as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility and matrix invasion give it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Product Specifications

Synonyms

C230052L06Rik Protein; Hgf Protein; HGF/SF Protein; NK1 Protein; NK2 Protein; SF Protein; SF/HGF Protein

Accession Number

NP_000592.3

Reactivity

Human

Cross Reactivity

Human

Assay Protocol

See Datasheet send with the product

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 0.2-2 ng/mL. 2.Immobilized Recombinant Human HGF / Hepatocyte Growth Factor Protein at 2μg/mL (100μL/well) can bind Recombinant Human c-MET / HGFR Protein (His Tag), the EC50 is 16-48 ng/mL. 3.Human cholangiocarcinomas organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, NOG, RSPO1. 4.Human liver cancer organoids were cultured with FGF2, HGF, FGF7, EGF, FGF10, TGFB1, NOG, RSPO1. 5.Human kidney organoids were cultured with FGF7, EGF, FGF10, NOG, RSPO1, HGF, FGF4. 6.Western blot analysis of extracts from A549, untreated (line A) or treated with HGF (50 ng/mL, 5min) (line B), using Phospho-Met (Tyr1234, 1235) Rabbit monoclonal Antibody at 1:1000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal at 1:50000 dilution (lower) .

Length

697

Form

Lyophilized or frozen liquid

Buffer

PBS, 100mM Arg,0.05% Tween20, pH7.0.

Reconstitution

See Datasheet

Molecular Weight

90 kDa, 60 kDa and 34 kDa (glycosylated)

Storage Conditions

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.