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Growth Hormone

Recombinant rat GH-22K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22367 Dalton.

Product Specifications

Synonyms

Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

NCBI Gene ID

24391

UniProt

P01244

Accession Number

NP_001030020.2

Accession Number mRNA

NM_001034848.2

Reactivity

Rat

Sequence

AAFPAMPLSSLFANAVLRAQHLHQLAADTYKEFERAYIPEGQRYSIQNAQAAFCFSETIPAPTGKEEAQQRTDMELLRFSLLLIQSWLGPVQFLSRIFTNSLMFGTSDRVYEKLKDLEEGIQALMQELEDGSPRIGQILKQTYDKFDANMRSDDALLKNYGLLSCFKKDLHKAETYLRVMKCRRFAESSCAF

Assay Protocol

It is recommended to reconstitute the lyophilized rGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98.0% as determined by Gel filtration and SDS-PAGE gel.

Bioactivity

RGH is fully biologically active when compared to WHO reference standard using in vitro bioassay. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) .

Length

192

Form

Lyophilized

Reconstitution

Water, pH 9

Molecular Weight

22.3 kDa

Host or Source

E. coli

N Terminal Sequence

AFPAM

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