Growth Hormone
Recombinant rat GH-22K produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 22367 Dalton.
Product Specifications
Synonyms
Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone
NCBI Gene ID
24391
UniProt
P01244
Accession Number
NP_001030020.2
Accession Number mRNA
NM_001034848.2
Reactivity
Rat
Sequence
AAFPAMPLSSLFANAVLRAQHLHQLAADTYKEFERAYIPEGQRYSIQNAQAAFCFSETIPAPTGKEEAQQRTDMELLRFSLLLIQSWLGPVQFLSRIFTNSLMFGTSDRVYEKLKDLEEGIQALMQELEDGSPRIGQILKQTYDKFDANMRSDDALLKNYGLLSCFKKDLHKAETYLRVMKCRRFAESSCAF
Assay Protocol
It is recommended to reconstitute the lyophilized rGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.
Endotoxin
< 0.1 ng/µg of protein (< 1EU/µg)
Purity
> 98.0% as determined by Gel filtration and SDS-PAGE gel.
Bioactivity
RGH is fully biologically active when compared to WHO reference standard using in vitro bioassay. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) .
Length
192
Form
Lyophilized
Reconstitution
Water, pH 9
Molecular Weight
22.3 kDa
Host or Source
E. coli
N Terminal Sequence
AFPAM
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