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Growth Hormone

Recombinant ovine growth hormone, one polypeptide chain containing 191 amino acids and having a molecular mass of ~ 21.8 kDa.

Product Specifications

Synonyms

Somatotropin, GH, GH-N, Growth Hormone 1, Pituitary growth hormone

NCBI Gene ID

443329

UniProt

P67930

Accession Number

NP_001009315.2

Accession Number mRNA

NM_001009315.3

Reactivity

Ovine

Sequence

AFPAMSLSGLFANAVLRAQHLHQLAADTFKEFERTYIPEGQRYSIQNTQVAFCFSETIPAPTGKNEAQQKSDLELLRISLLLIQSWLGPLQFLSRVFTNSLVFGTSDRVYEKLKDLEEGILALMRELEDVTPRAGQILKQTYDKFDTNMRSDDALLKNYGLLSCFRKDLHKTETYLRVMKCRRFGEASCAF

Assay Protocol

It is recommended to reconstitute the lyophilized oGH in 0.4% NaHCO3 or water adjusted to pH 9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier protein such as BSA or similar.

Endotoxin

< 0.1 ng/µg of protein (< 1EU/µg)

Purity

> 98.0% as determined by RP-HPLC, Gel filtration and SDS-PAGE Silver Stained gel.

Bioactivity

OGH is fully biologically active when compared to WHO reference standard using in vitro bioassay in PDF-P1 3B9 cells stably transfected with rabbit GH receptors. It is also capable of forming a 1:2 complex with the recombinant ovine growth hormone receptor extracellular domain (ECD) .

Length

191

Form

Lyophilized

Reconstitution

Water

Molecular Weight

21.8 kDA

Host or Source

E. coli

N Terminal Sequence

ATFPA

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